MDF is a conserved splicing factor and modulates cell division and stress response in Arabidopsis.

ABSTRACT: The coordination of cell division with stress response is essential for maintaining genome stability in plant meristems. Proteins involved in pre-mRNA splicing are important for these processes in animal and human cells. Based on its homology to the splicing factor SART1, which is implicated in the control of cell division and genome stability in human cells, we analyzed if MDF has similar functions in plants. We found that MDF associates with U4/U6.U5 tri-snRNP proteins and is essential for correct splicing of 2,037 transcripts. Loss of MDF function leads to cell division defects and cell death in meristems and was associated with up-regulation of stress-induced genes and down-regulation of mitotic regulators. In addition, the mdf-1 mutant is hypersensitive to DNA damage treatment

SUBMITTER: de Luxan-Hernandez C 

PROVIDER: S-EPMC9585968 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature