Proximity proteomics of synaptopodin provides insight into the molecular composition of the spine apparatus of dendritic spines.

ABSTRACT: The spine apparatus is a specialized compartment of the neuronal smooth endoplasmic reticulum (ER) located in a subset of dendritic spines. It consists of stacks of ER cisterns that are interconnected by an unknown dense matrix and are continuous with each other and with the ER of the dendritic shaft. While this organelle was first observed over 60 y ago, its molecular organization remains a mystery. Here, we performed in vivo proximity proteomics to gain some insight into its molecular components. To do so, we used the only known spine apparatus-specific protein, synaptopodin, to target a biotinylating enzyme to this organelle. We validated the specific localization in dendritic spines of a small subset of proteins identified by this approach, and we further showed their colocalization wi

SUBMITTER: Falahati H 

PROVIDER: S-EPMC9586327 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature