Dynamic changes in chromatin accessibility are associated with the atherogenic transitioning of vascular smooth muscle cells.

ABSTRACT:

Aims

De-differentiation and activation of pro-inflammatory pathways are key transitions vascular smooth muscle cells (SMCs) make during atherogenesis. Here, we explored the upstream regulators of this 'atherogenic transition'.

Methods and results

Genome-wide sequencing studies, including Assay for Transposase-Accessible Chromatin using sequencing and RNA-seq, were performed on cells isolated from both murine SMC-lineage-tracing models of atherosclerosis and human atherosclerotic lesions. At the bulk level, alterations in chromatin accessibility were associated with the atherogenic transitioning of lesional SMCs, especially in relation to genes that govern differentiation status and complement-dependent inflammation. Using computational biology, we observed that a transcripti

SUBMITTER: Wang Y 

PROVIDER: S-EPMC9586565 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature