A localized calcium transient and polar body abscission.

ABSTRACT: Polar body emission is a special form of cytokinesis in oocyte meiosis that ensures the correct number of chromosomes in reproduction-competent eggs. The molecular mechanism of the last step, polar body abscission, is poorly understood. While it has been proposed that Ca²⁺ signaling plays important roles in embryonic cytokinesis, to date transient increases in intracellular free Ca²⁺ have been difficult to document in oocyte meiosis except for the global Ca²⁺ wave induced by sperm at fertilization. Here, we find that microinjection of the calcium chelator dibromo-BAPTA inhibits polar body abscission in Xenopus laevis oocytes. Using a novel, microtubule-targeted ratio-metric calcium sensor, we detected a calcium transient that is focused at the contra

SUBMITTER: Mo G 

PROVIDER: S-EPMC9586590 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature