Dataset Information

Chloroplast genome characterization of Rubus arcticus L.

ABSTRACT: Rubus arcticus Linnaeus (1753) is a medicinal and edible plant in the Rosaceae with wide distribution in northeast China. The total length of the genome was 156,668 bp with a GC content of 37.1%, including a large single-copy (LSC, 85,958 bp) region, a small single-copy region (SSC, 18,756 bp), and inverted repeat (IR, 51,954 bp) regions. A total of 129 genes were identified. The numbers of protein genes tRNAs and rRNAs were 85, 36, and 8, respectively. Phylogenetic analysis indicated that R. arcticus belongs to the Rubus genus. Published R. arcticus chloroplast genomes have yielded insights into the closely related species identification, phylogenetic position and Rubus evolution.

SUBMITTER: Jiang Z

PROVIDER: S-EPMC9586667 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Chloroplast genome characterization of <i>Rubus arcticus</i> L.

Jiang Zhehui Z Wang Tianhao T Gao Yuan Y Shu Dingxi D Li Xiangquan X Ren Weichao W Ma Wei W Bao Yihong Y

Mitochondrial DNA. Part B, Resources 20221018 10

<i>Rubus arcticus</i> Linnaeus (1753) is a medicinal and edible plant in the Rosaceae with wide distribution in northeast China. The total length of the genome was 156,668 bp with a GC content of 37.1%, including a large single-copy (LSC, 85,958 bp) region, a small single-copy region (SSC, 18,756 bp), and inverted repeat (IR, 51,954 bp) regions. A total of 129 genes were identified. The numbers of protein genes tRNAs and rRNAs were 85, 36, and 8, respectively. Phylogenetic analysis indicated tha ...[more]

PMID: 36278129

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Project description:Background and aimsPreservation of cultivar purity creates a particular challenge for plants that are self-incompatible, require insects for cross-pollination, and have easily germinating seeds and vigorously spreading rhizomes. As the fields must be planted with mixed populations, and a balance must be maintained between the cultivars to achieve effective pollination, methods for field monitoring of the relative density of different cultivars must be practical. Furthermore, a DNA-based method is needed for cultivar verification in the collections and outside of the growing season. The aim of this study was to develop both types of methods for Rubus arcticus (arctic bramble).MethodsMorphological parameters were measured from six cultivars grown on three farms. Observations from the flowers and fruits included: petal and sepal number, flower diameter, arrangement of petals, size of calyx in relation to corolla, fruit weight, yield and soluble sugars. Observations from the leaves included: width and height of middle leaflet, shape of the base of terminal leaflet, shape of terminal leaflet, leaf margin serration and fingertip touch. The applicability of simple sequence repeat (SSR) or microsatellite DNA markers developed for red raspberry was tested on eight arctic bramble cultivars.Key results and conclusionsMorphological and molecular identification methods were developed for R. arcticus. The best morphological characteristics were the length-to-width ratio of the middle leaflet and leaf margin serration. A particular characteristic, fingertip touch, was shown by electron microscopy to be related to the density and quality of the leaf hairs. Red raspberry SSR marker no. 126 proved to be applicable for differentiation of the eight arctic bramble cultivars tested. These identification methods are critical to secure the maintenance and management of R. arcticus. However, the challenges faced and approaches taken are equally applicable to other species with similar biology.

Dataset Information

Chloroplast genome characterization of Rubus arcticus L.

Publications

Chloroplast genome characterization of <i>Rubus arcticus</i> L.

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