Investigating lytic polysaccharide monooxygenase-assisted wood cell wall degradation with microsensors.

ABSTRACT: Lytic polysaccharide monooxygenase (LPMO) supports biomass hydrolysis by increasing saccharification efficiency and rate. Recent studies demonstrate that H₂O₂ rather than O₂ is the cosubstrate of the LPMO-catalyzed depolymerization of polysaccharides. Some studies have questioned the physiological relevance of the H₂O₂-based mechanism for plant cell wall degradation. This study reports the localized and time-resolved determination of LPMO activity on poplar wood cell walls by measuring the H₂O₂ concentration in their vicinity with a piezo-controlled H₂O₂ microsensor. The investigated Neurospora crassa LPMO binds to the inner cell wall layer and consumes enzymatically generated H₂O<

SUBMITTER: Chang H 

PROVIDER: S-EPMC9586961 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature