Reference genes for the developing mouse lung under consideration of biological, technical and experimental confounders.

ABSTRACT: For gene expression analysis, the raw data obtained from RT-qPCR are preferably normalized to reference genes, which should be constantly expressed regardless of experimental conditions. Selection of reference genes is particularly challenging for the developing lung because of the complex transcriptional and epigenetic regulation of genes during organ maturation and injury repair. To date, there are only limited experimental data addressing reliable reference genes for this biological circumstance. In this study, we evaluated reference genes for the lung in neonatal C57BL/6 mice under consideration of biological, technical and experimental conditions. For that, we thoroughly selected candidates from commonly used reference genes side-by-side with novel ones by analyzing publicly available

SUBMITTER: Shin H 

PROVIDER: S-EPMC9587035 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature