Modelling reoxygenation effects in non-small cell lung cancer cell lines and showing epithelial-mesenchymal transition.

ABSTRACT:

Purpose

Circulating tumour cells (CTCs) are a rare cell subpopulation regulated by the tumour microenvironment. In hypoxic conditions, CTCs are able to invade the lymphatic and circulatory systems leading to metastasis at distant sites.

Methods

To mimic in vivo oxygen variations and effects on CTCs, we have cultured five non-small cell lung cancer (NSCLC) cell lines under normoxic and hypoxic conditions, followed by a pulse of reoxygenation for 4 h.

Results

Proliferation, spheroid-formation and colony formation ability under varying O₂ levels were investigated. Proliferation rate was not altered when cells were cultured in 2D models under hypoxic conditions. However, we observed that hypoxia enhanced in vitro formation of tumour-spheres and accelerated clon

SUBMITTER: Kapeleris J 

PROVIDER: S-EPMC9587087 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature