Heterogeneity of ferrous iron-containing endolysosomes and effects of endolysosome iron on endolysosome numbers, sizes, and localization patterns.

ABSTRACT: Endolysosomes are key regulators of iron metabolism and are central to iron trafficking and redox signaling. Iron homeostasis is linked to endolysosome acidity and inhibition of endolysosome acidity triggers iron dysregulation. Because of the physiological importance and pathological relevance of ferrous iron (Fe²⁺ ), we determined levels of Fe²⁺ specifically and quantitatively in endolysosomes as well as the effects of Fe²⁺ on endolysosome morphology, distribution patterns, and function. The fluorescence dye FeRhoNox-1 was specific for Fe²⁺ and localized to endolysosomes in U87MG astrocytoma cells and primary rat cortical neurons; in U87MG cells the endolysosome concentration of Fe²⁺ ([Fe²⁺ ]_el ) was 50.4 μM in c

SUBMITTER: Halcrow PW 

PROVIDER: S-EPMC9587899 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature