Proteome alterations during clonal isolation of established human pancreatic cancer cell lines.

ABSTRACT: Clonal isolation is an integral step of numerous workflows in genome editing and cell engineering. It comprises the isolation of a single progenitor cell from a defined cell line population with subsequent expansion to obtain a monoclonal cell population. This process is associated with transient loss of cell-cell contacts and absence of a multicellular microenvironment. Previous studies have revealed transcriptomic changes upon clonal isolation with cell line specific extent. Since transcriptome alterations are only partially reflected on the proteome level, we sought to investigate the impact of clonal isolation on the cellular proteome to a depth of > 6000 proteins in three established pancreatic cancer cell lines. We show that clonal isolation does have an impact on the cellular proteo

SUBMITTER: Bernhard P 

PROVIDER: S-EPMC9587952 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature