Heterogeneity and evolution of tumour immune microenvironment in metastatic gastroesophageal adenocarcinoma.

ABSTRACT:

Background

Tumour immune microenvironment heterogeneity is prevalent in numerous cancers and can negatively impact immunotherapy response. Immune heterogeneity and evolution in gastroesophageal adenocarcinoma (GEA) have not been studied in the past.

Methods

Together with a multi-region sampling of normal, primary and metastatic tissues, we performed whole exome sequencing, TCR sequencing as well as immune cell infiltration estimation through deconvolution of gene expression signals.

Results

We discovered high TCR repertoire and immune cell infiltration heterogeneity among metastatic sites, while they were homogeneous among primary and normal samples. Metastatic sites shared high levels of abundant TCR clonotypes with blood, indicating immune surveillance via blood. Me

SUBMITTER: Wang W 

PROVIDER: S-EPMC9587966 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature