Nanoscale analysis of frozen honey by atom probe tomography.

ABSTRACT: Three-dimensional reconstruction of the analysed volume is one of the main goals of atom probe tomography (APT) and can deliver nearly atomic resolution (~ 0.2 nm spatial resolution) and chemical information with a mass sensitivity down to the ppm range. Extending this technique to frozen biological systems would have an enormous impact on the structural analysis of biomolecules. In previous works, we have shown that it is possible to measure frozen liquids with APT. In this paper, we demonstrate the ability of APT to trace nanoscale precipitation in frozen natural honey. While the mass signals of the common sugar fragments C_xH_y and C_xO_yH_z overlap with (H₂O)_nH from water, we achieved correct stoichiometric values

SUBMITTER: Schwarz TM 

PROVIDER: S-EPMC9587987 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature