Single-cell sequencing deconvolutes cellular responses to exercise in human skeletal muscle.

ABSTRACT: Skeletal muscle adaptations to exercise have been associated with a range of health-related benefits, but cell type-specific adaptations within the muscle are incompletely understood. Here we use single-cell sequencing to determine the effects of exercise on cellular composition and cell type-specific processes in human skeletal muscle before and after intense exercise. Fifteen clusters originating from six different cell populations were identified. Most cell populations remained quantitatively stable after exercise, but a large transcriptional response was observed in mesenchymal, endothelial, and myogenic cells, suggesting that these cells are specifically involved in skeletal muscle remodeling. We found three subpopulations of myogenic cells characterized by different maturation stages

SUBMITTER: Lovric A 

PROVIDER: S-EPMC9588010 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature