Large-volume and deep brain imaging in rabbits and monkeys using COMPACT two-photon microscopy.

ABSTRACT: In vivo imaging has been widely used for investigating the structure and function of neurons typically located within ~ 800 μm below the cortical surface. Due to light scattering and absorption, it has been difficult to perform in-vivo imaging of neurons in deep cortical and subcortical regions of large animals with two-photon microscopy. Here, we combined a thin-wall quartz capillary with a GRIN lens attached to a prism for large-volume structural and calcium imaging of neurons located 2 mm below the surface of rabbit and monkey brains. The field of view was greatly expanded by rotating and changing the depth of the imaging probe inside a quartz capillary. Calcium imaging of layer 5/6 neurons in the rabbit motor cortex revealed differential activity of these neurons between quiet wakefuln

SUBMITTER: Lu Y 

PROVIDER: S-EPMC9588025 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature