Comparative Study of The Effects of Confounding Factors on Improving Rat Pancreatic Islet Isolation Yield and Quality.

ABSTRACT:

Objective

Isolated pancreatic islets are valuable resources for a wide range of research, including cell replacement studies and cell-based platforms for diabetes drug discovery and disease modeling. Islet isolation is a complex and stepwise procedure aiming to obtain pure, viable, and functional islets for in vitro and in vivo studies. It should be noted that differences in rodent strains, gender, weight, and density gradients may affect the isolated islet's properties. We evaluated the variables affecting the rat islet isolation procedure to reach the maximum islet yield and functionality, which would be critical for further studies on islet regenerative biology.

Materials and methods

The present experimental study compared the yield and purity of isolated islets from nond

SUBMITTER: Moazenchi M 

PROVIDER: S-EPMC9588161 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature