Quantitative analysis of transcriptome dynamics provides novel insights into developmental state transitions.

ABSTRACT:

Background

During embryogenesis, the developmental potential of initially pluripotent cells becomes progressively restricted as they transit to lineage restricted states. The pluripotent cells of Xenopus blastula-stage embryos are an ideal system in which to study cell state transitions during developmental decision-making, as gene expression dynamics can be followed at high temporal resolution.

Results

Here we use transcriptomics to interrogate the process by which pluripotent cells transit to four different lineage-restricted states: neural progenitors, epidermis, endoderm and ventral mesoderm, providing quantitative insights into the dynamics of Waddington's landscape. Our findings provide novel insights into why the neural progenitor state is the default lineage state fo

SUBMITTER: Johnson K 

PROVIDER: S-EPMC9588240 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature