Mesenchymal stem cells cause induction of granulocyte differentiation of rat bone marrow C-kit⁺ hematopoietic stem cells through JAK3/STAT3, ERK, and PI3K signaling pathways.

ABSTRACT:

Objectives

Hematopoietic stem cells (HSCs) are the cells that give rise to different types of blood cells during the hematopoiesis process. Mesenchymal stromal cells (MSCs) as key elements in the bone marrow (BM) niche interact with hematopoietic progenitor cells (HPCs) by secreting cytokines, which control HPCs maintenance and fate. Here we report that BM-MSCs are capable of inducing granulocytic differentiation of the C-Kit+ HSCs via activating JAK3/STAT3, ERK, and PI3K signaling pathways.

Materials and methods

For this purpose, BM-MSCs and C-kit⁺ HSCs were isolated. Next, cells were divided into two groups and differentiated into granulocytes: C-kit⁺ HSCs alone (control group) and co-cultured C-kit⁺ HSCs with MSCs (experimental group). Aft

SUBMITTER: Fathi E 

PROVIDER: S-EPMC9588313 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature