Nanoarchitecture and molecular interactions of epithelial cell junction proteins revealed by super-resolution microscopy.

ABSTRACT: Epithelial cells are polarized with defined apical tight junctions (TJs), lateral adherens junctions (AJs), and basal integrin-matrix interactions. However, it is increasingly recognized that resident cell junction proteins can be found in varying locations and with previously unrecognized functions. Our study here presents the nanoarchitecture and nanocolocalization of cell junction proteins in culture and tissue by stochastic optical reconstruction microscopy (STORM). The Z-axial view of noncancerous MDCK-II and PZ-HPV-7 cell-cell junctions resolved β-catenin and p120^ctn localizations to TJs and AJs, with p120^ctn apical to β-catenin and colocalizing with TJ protein claudin-7. More basally, p120^ctn and β-catenin become colocalized. This topography was lost

SUBMITTER: Naser AN 

PROVIDER: S-EPMC9588527 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature