EWS::FLI1 and HOXD13 Control Tumor Cell Plasticity in Ewing Sarcoma.

ABSTRACT:

Purpose

Propagation of Ewing sarcoma requires precise regulation of EWS::FLI1 transcriptional activity. Determining the mechanisms of fusion regulation will advance our understanding of tumor progression. Here we investigated whether HOXD13, a developmental transcription factor that promotes Ewing sarcoma metastatic phenotypes, influences EWS::FLI1 transcriptional activity.

Experimental design

Existing tumor and cell line datasets were used to define EWS::FLI1 binding sites and transcriptional targets. Chromatin immunoprecipitation and CRISPR interference were employed to identify enhancers. CUT&RUN and RNA sequencing defined binding sites and transcriptional targets of HOXD13. Transcriptional states were investigated using bulk and single-cell transcriptomic data from cell

SUBMITTER: Apfelbaum AA 

PROVIDER: S-EPMC9588607 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature