Wound swabs versus biopsies to detect methicillin resistant Staphylococcus aureus in experimental equine wounds.

ABSTRACT:

Objective

To compare: (1) the load and diversity of cultivatable bacterial species isolated from tissue biopsies with cultures from surface swabs, and (2) the ability of each technique to detect methicillin-resistant Staphylococcus aureus (MRSA) in a model of MRSA-infected equine wounds.

Study design

Experimental in vivo study.

Animals

Three light-breed adult horses.

Methods

Four 2.5 × 2.5 cm full-thickness skin wounds were created on the dorsolateral aspect of each forelimb. Five days later, each wound was inoculated with a pure culture of MRSA (ATCC 43300). One hundred microlitres of 0, 5 × 10⁸ , 5 × 10⁹ or 5 × 10¹⁰ colony forming units (CFU)/ml was used to inoculate each wound. Surface swabs (Levine technique) and tissue biop

SUBMITTER: Brock AK 

PROVIDER: S-EPMC9588683 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature