LC-MS/MS for Assessing the Incorporation and Repair of N²-Alkyl-2'-deoxyguanosine in Genomic DNA.

ABSTRACT: Understanding the occurrence, repair, and biological consequences of DNA damage is important in environmental toxicology and risk assessment. The most common way to assess DNA damage elicited by exogenous sources in a laboratory setting is to expose cells or experimental animals with chemicals that modify DNA. Owing to the lack of reaction specificities of DNA damaging agents, the approach frequently does not allow for induction of a specific DNA lesion. Herein, we employed metabolic labeling to selectively incorporate N²-methyl-dG (N²-MedG) and N²-n-butyl-dG (N²-nBudG) into genomic DNA of cultured mammalian cells, and investigated how the levels of the two lesions in cellular DNA are modulated by differe

SUBMITTER: Guo S 

PROVIDER: S-EPMC9588702 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature