Integrating network pharmacology and experimental verification to explore the mechanism of puerarin against oliguria in acute alcoholism.

ABSTRACT: Purpose: This study was designed to evaluate the pharmacological mechanisms of puerarin against oliguria in acute alcoholism via network pharmacology analysis combined with experimental verification. Methods: First, this study established an acute alcoholism rat model, compared the changes in urine volume in each group, and observed the therapeutic effect of puerarin by H&E staining, biochemical, RT-qPCR, and immunohistochemical analyses. Second, puerarin-related targets were searched in TCMS, PubChem, CNKI, Wanfang, PubMed, and GeenMedical Academic databases. Also, potential disease targets were obtained from the GeneCards, MalaCards, and NCBI-gene databases and genes with puerarin target gene intersections were screened out. The interaction network for co-predicted t

SUBMITTER: Wan MX 

PROVIDER: S-EPMC9589237 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature