Claudin-18 expression under hyperoxia in neonatal lungs of bronchopulmonary dysplasia model rats.

ABSTRACT:

Background

Bronchopulmonary dysplasia (BPD) is characterized by impaired alveolar and microvascular development. Claudin-18 is the only known lung-specific tight junction protein affecting the development and transdifferentiation of alveolar epithelium.

Objective

We aimed to explore the changes in the expression of claudin-18, podoplanin, SFTPC, and the canonical WNT pathway, in a rat model of hyperoxia-induced BPD, and to verify the regulatory relationship between claudin-18 and the canonical WNT pathway by cell experiments.

Methods

A neonatal rat and cell model of BPD was established by exposing to hyperoxia (85%). Hematoxylin and eosin (HE) staining was used to confirm the establishment of the BPD model. The mRNA levels were assessed using quantitative real-time po

SUBMITTER: Zuo J 

PROVIDER: S-EPMC9589239 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature