Use of dielectrophoresis for directing T cells to microwells before nanostraw transfection: modelling and experiments.

ABSTRACT: Nanostraw substrates have great potential for achieving minimally invasive cell transfection. Cells located on the nanostraw substrate are subjected to mild DC electric pulses applied across the nanostraw substrate, which open pores in the cell membrane on top of the nanostraws and drives charged cargo through these pores via electrophoresis. However, with this method, the current may leak through uncovered nanostraws, thereby decreasing the desired effect in the cell-covered nanostraws. A minimization of the number of uncovered nanostraws could be achieved by high cell coverage, but this is challenging when working with small cell populations. Nanostraw substrates of smaller area could be covered by smaller cell populations but are hard to integrate into fluidics systems. Here, we

SUBMITTER: Lard M 

PROVIDER: S-EPMC9589401 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature