Evaluation and comparison of in vitro intrinsic clearance rates measured using cryopreserved hepatocytes from humans, rats, and rainbow trout.

ABSTRACT: In vitro and in silico methods that can reduce the need for animal testing are being used with increasing frequency to assess chemical risks to human health and the environment. The rate of hepatic biotransformation is an important species-specific parameter for determining bioaccumulation potential and extrapolating in vitro bioactivity to in vivo effects. One approach to estimating hepatic biotransformation is to employ in vitro systems derived from liver tissue to measure chemical (substrate) depletion over time which can then be translated to a rate of intrinsic clearance (CL_int). In the present study, cryopreserved hepatocytes from humans, rats, and rainbow trout were used to measure CL_int values for 54 industrial and pesticidal chemicals at starting test concent

SUBMITTER: Black SR 

PROVIDER: S-EPMC9589910 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature