Zebrafish Slit2 and Slit3 Act Together to Regulate Retinal Axon Crossing at the Midline.

ABSTRACT: Slit-Robo signaling regulates midline crossing of commissural axons in different systems. In zebrafish, all retinofugal axons cross at the optic chiasm to innervate the contralateral tectum. Here, the mutant for the Robo2 receptor presents severe axon guidance defects, which were not completely reproduced in a Slit2 ligand null mutant. Since slit3 is also expressed around this area at the stage of axon crossing, we decided to analyze the possibility that it collaborates with Slit2 in this process. We found that the disruption of slit3 expression by sgRNA-Cas9 injection caused similar, albeit slightly milder, defects than those of the slit2 mutant, while the same treatment in the slit2-/-^mz background caused much more severe defects, comparable to thos

SUBMITTER: Davison C 

PROVIDER: S-EPMC9590056 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature