Electrotransfer of Immunoprobes through Thin-Layer Polyacrylamide Gels.

ABSTRACT: Hydrogels are important structural and operative components of microfluidic systems, finding diverse utility in biological sample preparation and interrogation. One inherent challenge for integrating hydrogels into microfluidic tools is thermodynamic molecular partitioning, which reduces the in-gel concentration of molecular solutes (e.g., biomolecular regents), as compared to the solute concentration in an applied solution. Consequently, biomolecular reagent access to in-gel scaffolded biological samples (e.g., encapsulated cells, microbial cultures, target analytes) is adversely impacted in hydrogels. Further, biomolecular reagents are typically introduced to the hydrogel via diffusion. This passive process requires long incubation periods compared to active biomolecular delivery techniq

SUBMITTER: Mourdoukoutas AP 

PROVIDER: S-EPMC9590380 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature