Telomerase reverse transcriptase promotes angiogenesis in neonatal rats after hypoxic-ischemic brain damage.

ABSTRACT:

Background

Angiogenesis is an endogenous repair mechanism following hypoxic-ischemic brain damage (HIBD). Interestingly, recent studies have shown that angiogenesis can be regulated by telomerase reverse transcriptase (TERT), a critical component of telomerase. As telomerase reverse transcriptase can promote angiogenesis after stroke, we hypothesized that it could also promote angiogenesis after HIBD. To test this hypothesis, we developed in vivo and in vitro HIBD models in neonatal rats.

Methods

TERT was overexpressed by lentivirus and adenovirus infection, and levels were measured using quantitative real-time polymerase chain reaction. We used a cell counting kit to quantify the proliferation rate of brain microvascular endothelial cells (BMECs), and immunofl

SUBMITTER: Li J 

PROVIDER: S-EPMC9590416 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature