Phenotypic analysis of erythrocytes in sickle cell disease using imaging flow cytometry.

ABSTRACT: The morphology and other phenotypic characteristics of erythrocytes in sickle cell disease (SCD) have been analyzed for decades in patient evaluation. This involves a variety of techniques, including microscopic analysis of stained blood films, flow cytometry, and cell counting. Here, we analyzed SCD blood using imaging flow cytometry (IFC), a technology that combines flow cytometry and microscopy to enable simultaneous rapid-throughput analysis of cellular morphology and cell-surface markers. With IFC, we were able to automate quantification of poikilocytes from SCD blood. An important subpopulation of poikilocytes represented dense cells, although these could not be distinguished from other poikilocytes without first centrifuging the blood through density gradients. In addition, CD71-pos

SUBMITTER: Ozpolat T 

PROVIDER: S-EPMC9592074 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature