LINC01140 Targeting miR-452-5p/RGS2 Pathway to Attenuate Breast Cancer Tumorigenesis

ABSTRACT:

Background

LINC01140 has been known to be involved in various cancers. However, its underlying molecular mechanism in breast cancer (BC) needs further exploration.

Methods

The LINC01140, miR-452-5p, and RGS2 levels in BC cells and tissues were evaluated by means of RT-qPCR and western blotting. The variations in the biological functions of BC cells were analyzed through CCK-8, transwell, western blotting, and xenograft experiments to observe cell viability, migration, levels of apoptosis-related proteins (Bax and Bcl-2), and tumor growth. The correlations existing among LINC01140, miR-452-5p, and RGS2 were validated through luciferase reporter and RIP assays.

Results

LINC01140 and RGS2 were remarkably downregulated in BC cells and tissues, whereas miR-452-5p w

SUBMITTER: Li X 

PROVIDER: S-EPMC9592237 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature