CD14 signaling mediates lung immunopathology and mice mortality induced by Achromobacter xylosoxidans.

ABSTRACT:

Objective and design

Our research aimed to investigate the role of CD14 in pulmonary infection by Achromobacter xylosoxidans in an experimental murine model.

Methods

C57Bl/6 or CD14-deficient mice were infected intratracheally with non-lethal inoculum of A. xylosoxidans. At times 1, 3 and 7 days after infection, lungs, bronchoalveolar lavage and blood were collected. CD14 gene expression was determined by RT-PCR. The bacterial load in the lungs was assessed by counting colony forming units (CFU). Cytokines, chemokines, lipocalin-2 and sCD14 were quantified by the ELISA method. Inflammatory infiltrate was observed on histological sections stained with HE, and leukocyte subtypes were assessed by flow cytometry. In another set of experiments, C57Bl/6 or CD14-deficient mice were

SUBMITTER: Elias-Oliveira J 

PROVIDER: S-EPMC9592541 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature