Epitope of antiphospholipid antibodies retrieved from peptide microarray based on R39-R43 of β2-glycoprotein I.

ABSTRACT:

Background

Antiphospholipid antibody (aPL) syndrome (APS) is an autoimmune disease characterized by the presence of antiphospholipid antibodies and thromboembolic or pregnancy complications. Although cryptic epitope R39-R43 belonging to beta-2-glycoprotein 1 (β2GP1) has been identified as the main antigenic determinant for aPLs, we have recently demonstrated that the epitope is a motif determined by the polarity, rather than by the sequence or charge of amino acids.

Objective

In the present study, we wanted to identify the association of residues needed to obtain the highest aPL affinity.

Methods

Based on the epitope R39-R43 and our identified motif, we generated a printed peptide microarray of 676 different peptides. These peptides have been then screened for their a

SUBMITTER: Moghbel M 

PROVIDER: S-EPMC9592562 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature