High-level transient production of a protease-resistant mutant form of human basic fibroblast growth factor in Nicotiana benthamiana leaves.

ABSTRACT: The human basic fibroblast growth factor (bFGF) is a protein that plays a pivotal role in cellular processes like cell proliferation and development. As a result, it has become an important component in cell culture systems, with applications in biomedical engineering, cosmetics, and research. Alternative production techniques, such as transient production in plants, are becoming a feasible option as the demand continues to grow. High-level bFGF production was achieved in this study employing an optimized Agrobacterium-mediated transient expression system, which yielded about a 3-fold increase in production over a conventional system. This yield was further doubled at about 185 µg g^-1 FW using a mutant protease-resistant version that degraded/aggregated at a three-fold sl

SUBMITTER: Macauyag EA 

PROVIDER: S-EPMC9592933 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature