Establishment of an efficient transformation method of garden stock (Matthiola incana) using a callus formation chemical inducer.

ABSTRACT: Matthiola incana is an important floricultural plant that blooms from winter to spring, and had been desired to be established a transformation system. This study successfully obtained stable transgenic plants from M. incana. We used Agrobacterium tumefaciens harboring a binary vector containing the β-glucuronidase gene (GUS) under the control of cauliflower mosaic virus 35S promoter to evaluate the transformation frequency of M. incana. We observed that cocultivation with the A. tumefaciens strain GV3101 for 5 days effectively enhanced the infection frequency, assessed through a transient GUS expression area in the seedling. Furthermore, the addition of 100 µM acetosyringone was necessary for Agrobacterium infection. However, we coul

SUBMITTER: Tanahara Y 

PROVIDER: S-EPMC9592952 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature