CodY is modulated by YycF and affects biofilm formation in Staphylococcus aureus.

ABSTRACT:

Background

Staphylococcus aureus (S. aureus) is the leading cause of various infective diseases, including topical soft tissue infections. The goals of this study were to investigate the roles of YycF and CodY in the regulation of biofilm formation and pathogenicity.

Methods

Electrophoretic mobility shift assay (EMSA) was conducted to validate the bound promoter regions of YycF protein. We constructed the codY up-regulated or down-regulated S. aureus mutants. The biofilm biomass was determined by crystal violet microtiter assay and scanning electron microscopy (SEM). Quantitative RT-PCR analysis was used to detect the transcripts of biofilm-related genes. The live and dead cells of S. aureus biofilm were also investigated by confocal laser

SUBMITTER: Wu S 

PROVIDER: S-EPMC9593060 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature