Emergence of a floR-carrying plasmid in extended spectrum β-lactamase (ESBL) producing Pasteurella aerogenes, isolated from an avian species in China.

ABSTRACT: Identification and analysis of the antimicrobial resistance of Pasteurella aerogenes (P. aerogenes) isolated from poultry. For susceptibility testing in accordance with the CLSI, plasmids were extracted via alkaline lysis and transferred by CaCl₂ treatment. Genomic DNA of a representative P. aerogenes isolate was subjected to whole genome sequencing. CCCP was utilized to determine whether SF190908 contains an efflux pump. The bla_VEB gene was ligated with the pET-28 plasmid and transferred to Escherichia coli to verify it as an ESBL gene. SF190908 isolated from poultry was identified as P. aerogenes based upon biochemical and 16s rRNA results. The isolate showed high MIC values for eight antimicrobials. Sequencing results showed that the mobile element-mediated antimic

SUBMITTER: Yuan D 

PROVIDER: S-EPMC9593741 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature