Ontology highlight
ABSTRACT:
SUBMITTER: Pospisil S
PROVIDER: S-EPMC9594043 | biostudies-literature | 2022 Oct
REPOSITORIES: biostudies-literature
Pospíšil Šimon Š Panattoni Alessandro A Gracias Filip F Sýkorová Veronika V Hausnerová Viola Vaňková VV Vítovská Dragana D Šanderová Hana H Krásný Libor L Hocek Michal M
ACS chemical biology 20220609 10
Five 2'-deoxyribonucleoside triphosphates (dNTPs) derived from epigenetic pyrimidines (5-methylcytosine, 5-hydroxymethylcytosine, 5-formylcytosine, 5-hydroxymethyluracil, and 5-formyluracil) were prepared and systematically studied as substrates for nine DNA polymerases in competition with natural dNTPs by primer extension experiments. The incorporation of these substrates was evaluated by a restriction endonucleases cleavage-based assay and by a kinetic study of single nucleotide extension. All ...[more]