Organellar Glue: A Molecular Tool to Artificially Control Chloroplast-Chloroplast Interactions.

ABSTRACT: Organelles can physically interact to facilitate various cellular processes such as metabolite exchange. Artificially regulating these interactions represents a promising approach for synthetic biology. Here, we artificially controlled chloroplast-chloroplast interactions in living plant cells with our organelle glue (ORGL) technique, which is based on reconstitution of a split fluorescent protein. We simultaneously targeted N-terminal and C-terminal fragments of a fluorescent protein to the chloroplast outer envelope membrane or cytosol, respectively, which induced chloroplast-chloroplast interactions. The cytosolic C-terminal fragment likely functions as a bridge between two N-terminal fragments, thereby bringing the chloroplasts in close proximity to interact. We modulated the frequency

SUBMITTER: Ichikawa S 

PROVIDER: S-EPMC9594315 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature