Cystine-dependent antiporters buffer against excess intracellular reactive sulfur species-induced stress.

ABSTRACT: Reactive sulfur species (RSS) play a role in redox homeostasis; however, adaptive cell responses to excessive intracellular RSS are not well understood. Therefore, in this study, we generated transgenic (Tg) mice overexpressing cystathionine gamma-lyase (CSE) to produce excessive RSS. Contrary to expectations, tissue concentrations of RSS, such as cysteine persulfide (CysSSH), were comparable in both wild-type and CSE Tg mice, but the plasma concentrations of CysSSH were significantly higher in CSE Tg mice than in wild-type mice. This export of surplus intracellular RSS was also observed in primary hepatocytes of CSE Tg mice. Exposure of primary hepatocytes to the RSS generator sodium tetrasulfide (Na₂S₄) resulted in an initial increase in the intracellular concentrat

SUBMITTER: Akiyama M 

PROVIDER: S-EPMC9594640 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature