Precise Genomic Riboregulator Control of Metabolic Flux in Microbial Systems.

ABSTRACT: Engineered microbes can be used for producing value-added chemicals from renewable feedstocks, relieving the dependency on nonrenewable resources such as petroleum. These microbes often are composed of synthetic metabolic pathways; however, one major problem in establishing a synthetic pathway is the challenge of precisely controlling competing metabolic routes, some of which could be crucial for fitness and survival. While traditional gene deletion and/or coarse overexpression approaches do not provide precise regulation, cis-repressors (CRs) are RNA-based regulatory elements that can control the production levels of a particular protein in a tunable manner. Here, we describe a protocol for a generally applicable fluorescence-activated cell sorting technique used to isolate eight s

SUBMITTER: Pandey N 

PROVIDER: S-EPMC9594778 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature