Tissue clearing of human iPSC-derived organ-chips enables high resolution imaging and analysis.

ABSTRACT: Engineered microfluidic organ-chips enable increased cellular diversity and function of human stem cell-derived tissues grown in vitro. These three dimensional (3D) cultures, however, are met with unique challenges in visualization and quantification of cellular proteins. Due to the dense 3D nature of cultured nervous tissue, classical methods of immunocytochemistry are complicated by sub-optimal light and antibody penetrance as well as image acquisition parameters. In addition, complex polydimethylsiloxane scaffolding surrounding the tissue of interest can prohibit high resolution microscopy and spatial analysis. Hyperhydration tissue clearing methods have been developed to mitigate similar challenges of in vivo tissue imaging. Here, we describe an adaptation of this approac

SUBMITTER: Ondatje BN 

PROVIDER: S-EPMC9595176 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature