Nanovector-mediated exogenous delivery of dsRNA induces silencing of target genes in very young tomato flower buds.

ABSTRACT: RNA interference (RNAi) is a post-translational regulatory mechanism that controls gene expression in plants. This process can be artificially induced by double-stranded RNA (dsRNA) molecules with sequence homology to target mRNAs. Exogenously applied dsRNA on leaves has been shown to silence virulence genes of fungi and viruses, conferring protection to plants. Coupling dsRNA to nanoparticles has been demonstrated to prolong the silencing effect. The ability of exogenous dsRNA to silence endogenous genes in plants is currently under debate, mainly due to the difficulty in delivering dsRNA into plant tissues and organs. Our study aims to develop a method based on the exogenous application of dsRNA on tomato flowers for silencing endogenous genes controlling ovary growth. Two methods of dsR

SUBMITTER: Molesini B 

PROVIDER: S-EPMC9595187 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature