Profiling subcellular localization of nuclear-encoded mitochondrial gene products in zebrafish.

ABSTRACT: Most mitochondrial proteins are encoded by nuclear genes, synthetized in the cytosol and targeted into the organelle. To characterize the spatial organization of mitochondrial gene products in zebrafish (Danio rerio), we sequenced RNA from different cellular fractions. Our results confirmed the presence of nuclear-encoded mRNAs in the mitochondrial fraction, which in unperturbed conditions, are mainly transcripts encoding large proteins with specific properties, like transmembrane domains. To further explore the principles of mitochondrial protein compartmentalization in zebrafish, we quantified the transcriptomic changes for each subcellular fraction triggered by the chchd4a ^-/- mutation, causing the disorders in the mitochondrial protein import. Our result

SUBMITTER: Uszczynska-Ratajczak B 

PROVIDER: S-EPMC9595208 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature