Differential gene expression response of synovial fibroblasts from temporomandibular joints and knee joints to dynamic tensile stress.

ABSTRACT:

Purpose

Apart from other risk factors, mechanical stress on joints can promote the development of osteoarthritis (OA), which can also affect the temporomandibular joint (TMJ), resulting in cartilage degeneration and synovitis. Synovial fibroblasts (SF) play an important role in upkeeping joint homeostasis and OA pathogenesis, but mechanical stress as a risk factor might act differently depending on the type of joint. We thus investigated the relative impact of mechanical stress on the gene expression pattern of SF from TMJs and knee joints to provide new insights into OA pathogenesis.

Methods

Primary SF isolated from TMJs and knee joints of mice were exposed to mechanical strain of varying magnitudes. Thereafter, the expression of marker genes of the extracellular matrix (EC

SUBMITTER: Nazet U 

PROVIDER: S-EPMC9596579 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature