Functional Phosphoproteomics in Cancer Chemoresistance Using CRISPR-Mediated Base Editors.

ABSTRACT: Selective inhibition of targeted protein kinases is an effective therapeutic approach for treatment of human malignancies, which interferes phosphorylation of cellular substrates. However, a drug-imposed selection creates pressures for tumor cells to acquire chemoresistance-conferring mutations or activating alternative pathways, which can bypass the inhibitory effects of kinase inhibitors. Thus, identifying downstream phospho-substrates conferring drug resistance is of great importance for developing poly-pharmacological and targeted therapies. To identify functional phosphorylation sites involved in 5-fluorouracil (5-FU) resistance during its treatment of colorectal cancer cells, CRISPR-mediated cytosine base editor (CBE) and adenine base editor (ABE) are utilized for functional screens

SUBMITTER: Li J 

PROVIDER: S-EPMC9596822 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature