Near-Infrared Spectroscopy Enables Arthroscopic Histologic Grading of Human Knee Articular Cartilage.

ABSTRACT:

Purpose

To develop the means to estimate cartilage histologic grades and proteoglycan content in ex vivo arthroscopy using near-infrared spectroscopy (NIRS).

Methods

In this experimental study, arthroscopic NIR spectral measurements were performed on both knees of 9 human cadavers, followed by osteochondral block extraction and in vitro measurements: reacquisition of spectra and reference measurements (proteoglycan content, and three histologic scores). A hybrid model, combining principal component analysis and linear mixed-effects model (PCA-LME), was trained for each reference to investigate its relationship with in vitro NIR spectra. The performance of the PCA-LME model was validated with ex vivo spectra before and after the exclusion of outlying spectra. Model performanc

SUBMITTER: Sarin JK 

PROVIDER: S-EPMC9596902 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature