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YAP signaling is involved in WDR1-regulated proliferation and migration of non-small-cell lung cancer cells.


ABSTRACT: As a major co-factor of F-actin depolymerization, WD-repeat domain 1 (WDR1) affects the cellular microenvironment by cytoskeleton remodeling, thereby influencing cell molecular behavior. Our previous study showed that WDR1 activates YAP (Yes-associated protein) signaling in non-small-cell lung cancer (NSCLC) cells, but the mechanism remains unclear. We discovered that knockdown WDR1 in NSCLC cells decreased the expression of YAP and the nucleus-to-cytoplasm ratio. Disruption of cortical stress by drugs significantly inhibited YAP nuclear trafficking and enhanced YAP phosphorylation. In WDR1-knockdown NSCLC cells, inhibition of Hippo pathway reduced the nuclear exclusion of YAP and phosphorylated YAP. Our data suggest that WDR1-mediated cortical stress might be involved in regulating YAP signaling, thereby affecting the proliferation and migration of NSCLC cells.

SUBMITTER: An R 

PROVIDER: S-EPMC9597210 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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YAP signaling is involved in WDR1-regulated proliferation and migration of non-small-cell lung cancer cells.

An Ran R   Wang Junyan J   Chen Xuan X   Xu Ruifeng R   Hu Jisheng J   Liu Zhongying Z   Wei Chanjuan C   Zhang Chenxi C   Yuan Baiyin B  

Experimental biology and medicine (Maywood, N.J.) 20220721 18


As a major co-factor of F-actin depolymerization, WD-repeat domain 1 (WDR1) affects the cellular microenvironment by cytoskeleton remodeling, thereby influencing cell molecular behavior. Our previous study showed that WDR1 activates YAP (Yes-associated protein) signaling in non-small-cell lung cancer (NSCLC) cells, but the mechanism remains unclear. We discovered that knockdown WDR1 in NSCLC cells decreased the expression of YAP and the nucleus-to-cytoplasm ratio. Disruption of cortical stress b  ...[more]

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