A versatile Agrobacterium-based plant transformation system for genetic engineering of diverse citrus cultivars.

ABSTRACT: Developing an efficient transformation system is vital in genetically engineering recalcitrant crops, particularly trees. Here, we outline an Agrobacterium tumefaciens-based stable plant transformation methodology for citrus genetic engineering. The process was optimized to suit the requirements of fourteen citrus varieties by establishing appropriate infection, co-cultivation, selection, and culture media conditions. The procedure includes transforming seedling-derived epicotyl segments with an A. tumefaciens strain, then selecting and regenerating transformed tissues. Transgenic shoots were further identified by a visual reporter (e.g., β-glucuronidase) and confirmed by Northern and Southern blot analysis. Transgene integrations among the transgenic lines ranged between one

SUBMITTER: Dominguez MM 

PROVIDER: S-EPMC9597469 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature