Establishment of a stable, effective and universal genetic transformation technique in the diverse species of Brassica oleracea.

ABSTRACT: Brassica oleracea is an economically important species, including seven cultivated variants. Agrobacterium-mediated transformation of B. oleracea crops, mainly via hypocotyl and cotyledon, has been achieved in the past. However, previously established transformation methods showed low efficiency, severe genotype limitation and a prolonged period for transformants acquisition, greatly restricting its application in functional genomic studies and crop improvement. In this study, we have compared the shoot regeneration and genetic transformation efficiency of hypocotyl, cotyledon petiole and curd peduncle explants from twelve genotypes of cauliflower and broccoli. Finally, an Agrobacterium-mediated transformation method using curd peduncle as explant was est

SUBMITTER: Sheng X 

PROVIDER: S-EPMC9597678 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature