MiR-183/96/182 Cluster Regulates the Development of Bovine Myoblasts through Targeting FoxO1.

ABSTRACT: Muscle development is an important factor affecting meat yield and quality and is coordinated by a variety of the myogenic genes and signaling pathways. Recent studies reported that miRNA, a class of highly conserved small noncoding RNA, is actively involved in regulating muscle development, but many miRNAs still need to be further explored. Here, we identified that the miR-183/96/182 cluster exhibited higher expression in bovine embryonic muscle; meanwhile, it widely existed in other organizations. Functionally, the results of the RT-qPCR, EdU, CCK8 and immunofluorescence assays demonstrated that the miR-183/96/182 cluster promoted proliferation and differentiation of bovine myoblast. Next, we found that the miR-183/96/182 cluster targeted FoxO1 and restrained its expression. Meanw

SUBMITTER: Ru W 

PROVIDER: S-EPMC9597811 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature